Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 30;39:55. doi: 10.1186/s13046-020-01555-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — circFOXM1 facilitates cell proliferation by sponging miR-614. a RNA fluorescence in situ hybridization (FISH) showed that circFOXM1 was predominantly localized in cytoplasm in H1299 cells. U6 was mainly localized in nucleus. 18S was mainly localized in cytoplasm. b Cytoplasmic and nuclear RNA fractionation experiments showed that circFOXM1 was mainly located in the cytoplasm in H1299 cells. c Ago2 RIP assay was used for the detection of circFOXM1 in H1299 and H2170 cells expressing Flag-Ago2 or Flag-tag. d The putative binding sites of 17 predicted miRNAs on circFOXM1. e RNA pull-down assay was used for the detection of circFOXM1 by circFOXM1 specific probe. f-g Relative expression of circFOXM1 putative binding miRNAs was examined by qRT-PCR analysis by circFOXM1 specific probe. h Luciferase activity of luc-circFOXM1 wt or Luc-circFOXM1 mut in H1299 and H2170 cells co-transfected with miR-614 mimics. i-j Cell proliferation analysis of H1299 and H2170 cells transfected with control, circFOXM1, circFOXM1 + miR-NC (miRNA control), or circFOXM1 + miR-614. *P < 0.05; **P < 0.01