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. 2020 Mar 30;17:14. doi: 10.1186/s12950-020-00243-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Gene expression in purified classical, intermediate and non-classical monocyte subsets. a Dot plots with the mRNA expression levels of CX3CR1 and TNFα in classical, intermediate and non-classical monocytes purified from CCA (N = 8) and HCC (N = 20) patients, at T0 and T1, and from the HG (N = 10). Statistically significant differences were considered when p < 0.05; * between the groups indicated in the figure and the HG; # between T0 and T1. b Dot plots with the mRNA expression levels of TNFα in classical, intermediate and non-classical monocytes purified from CCA and HCC patients, at T0, comparing G1-G2 versus G3-G4 tumor histopathological grades. Statistically significant differences were considered when p < 0.05; * between the groups indicated in the figure