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. 2020 Mar 30;17:25. doi: 10.1186/s12987-020-00186-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Evaluation of TEER values in LN221F- and Matrigel-iBMELCs. a Scheme for the differentiation of iBMELCs. b Relative TEER values of 610B1-derived iBMELCs on day 10 on Matrigel, FBN, VTN-N, LN221F, LN411F, or LN511F. The relative TEER value of iBMELCs on Matrigel was defined as 1. Data are presented as the mean ± SD (n = 3; *p < 0.05, **p < 0.01; Tukey’s honestly significant difference test, Matrigel group vs. others). c Measurement of TEER values of LN221F- and Matrigel-iBMELCs derived from 610B1 and 648A1 over a long period (from day 9 to 18). Data are presented as the mean ± SD (n = 6; **p < 0.01; two-way repeated measures analysis of variance)