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. 2020 Mar 30;17:25. doi: 10.1186/s12987-020-00186-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Characterization of LN221F- and Matrigel-iBMELCs. a Relative mRNA expression levels of CDH5, MDR1, BCRP, ZO-1, occludin, GLUT1, and LAT1 in LN221F- and Matrigel-iBMELCs derived from 610B1 on day 10. The values are normalized to those of HPRT1. The relative mRNA expression levels of Matrigel-iBMELCs were defined as 1. Data are presented as the mean ± SD (n = 3; N.S. = not significant; Student’s t-test). hBMECs: n = 1. b Immunofluorescence analysis of CDH5 (orange), P-gp (orange), BCRP (orange), ZO-1 (orange), occludin (green), and claudin 5 (orange) expression in LN221F- and Matrigel-iBMELCs derived from 610B1 on day 10. DAPI = blue. Scale bars = 50 μm