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. 2020 Mar 30;20:262. doi: 10.1186/s12885-020-06762-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Up-regulated CHD4 was associated with a substantially poorer prognosis in NSCLC patients. a Representative IHC images of CHD4 in NSCLC and adjacent non-cancerous tissues. Left, magnification × 50; right, magnification × 200. b The IHC analysis of CHD4 in an independent set of paired NSCLC and matching non-tumor tissues; IHC signal intensities were scored as weak/negative or moderate/strong. The pie chart represented the proportions of NSCLC samples showing different intensities of IHC staining of CHD4. c The overall survival of 146 NSCLC patients with weak/negative or moderate/strong staining of CHD4. P < 0.05 was considered significant. IHC, immunohistochemistry