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. 2020 Mar 30;21:267. doi: 10.1186/s12864-020-6671-4

Table 6.

Primers of DEmRNAs

Gene name Forward primer (5′-3′) Reverse primer (5′-3′) Product length/bp Annealing temperature mRNA version
3BHSD agggcatctcagtggtca ggataaagactggcacgcta 144 57.4 °C NM_001285716.1
LEPR ccattgagaagtatcagttcagtc catgctggtgtttttcatcatcttg 105 58.4 °C XM_018045220.1
STAR cagaagggtgtcatcagagc tgagcagccaggtgagttt 97 58.6 °C XM_013975437.2
CCL21 ccgaaagaagattcccgcca ggcgagaacaggatagctgg 90 60.1 °C XM_005684096.3
RARRES1 gcgcgtgggttaatcagaag acattaacagctggtctgggtt 148 59.8 °C XM_018048385.1
DPT gtaccagacatgctccaacaa ctgttgtcagccagcaggaa 137 59.4 °C XM_005690613.3
β-actin tgcttctaggcggactgatt tacaatcaaagtcctcggccac 106 59.7 °C NM_001314342.1