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. 2020 Mar 24;8:144. doi: 10.3389/fcell.2020.00144

FIGURE 4.

FIGURE 4

Hepatic steatosis in phytol challenged PMP34 knockout mice. Liver sections of male control (WT) and knockout (KO) mice fed standard or phytol enriched diet were stained with Oil red O (ORO) (top row; scale bar 50 μm) or immunostained for macrophage marker F4/80 plus DAPI (second row; scale bar 200 μm; inset scale bar 25 μm), showing in the liver of the KO mice fed phytol accumulation of neutral lipids or enlarged macrophages in the process of phagocytosing cell debris (asterisks). Bottom, left: representative TLC of lipid extracts of liver of male age-matched WT and KO mice fed standard or phytol diet, visualized by iodine staining (solvent hexane/diethylether/acetic acid 70/30/1, v/v; portion of plate shown; direction of solvent indicated by arrow and its front by F). Standards, migration indicated, were cholesterol (C), triolein (triacylglycerides, TAG), cholesteryloleate (cholesterylester, CE); X indicates presence of new TAG species. When using hexane/diethylether/acetic acid (80/20/1, v/v) as solvent, spot X is splitting up in separate bands (right panel).