FIGURE 6.

Hepatic changes in phytol treated PMP34 knockout mice. Livers of adult male (M) or female (F) wild type (+/+) or knockout mice (–/–) given a 0.5% phytol enriched diet for 3 weeks were analyzed for protein and lipid content, fatty acid composition, and peroxisomal β-oxidation enzymes. Small amounts of branched fatty acids were present in all +/+ females, but only in one +/+ male animal detected (hence larger SD), whereas severe but variable accumulation was seen in all –/– animals. Arachidonic acid (C20:4) was expressed relative to the amount of palmitic acid (C16:0) per liver. ACOX1 values are several fold higher than in animals receiving a normal diet (see Supplementary Figure S3). Values are mean ± SD for 4-6 animals per gender and per genotype, treated in four different feeding studies. Values in heterozygous mice were similar to those in wild type animals (data not shown). Statistical differences are calculated per gender (Oneway ANOVA ***P < 0.005, **P < 0.01, *P < 0.05 KO versus WT). TAG, triacylglycerides; PL, phospholipid; 3MC13, 4,8,12-trimethyltridecanoic acid; mU, nmol/min.