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. 2020 Mar 24;8:144. doi: 10.3389/fcell.2020.00144

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Copyright © 2020 Van Veldhoven, de Schryver, Young, Zwijsen, Fransen, Espeel, Baes and Van Ael.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Total hepatic fatty acid profiles of phytol challenged PMP34 knockout mice. GC-MS profiles of tert-butyldimethylsilyl esters prepared from total lipid extracts from livers of age-matched male wild type (A) and knockout (B) mice fed phytol diet. A portion of the chromatogram (15-35 min) is shown. Major peaks are labeled for the wild type sample (A), and extra peaks appearing in the knockout sample are indicated in panel B. Using a temperature program better suited for separation of medium chain fatty acids, 2,6,10-trimethylundecanoic acid is also seen in the KO profiles (not shown) (IS, internal standard; tricosanoic acid, C23:0).