FIGURE 8.

Hepatic acyl-CoA profiles of phytol challenged PMP34 knockout mice. Panels (A,B) show representative RP-chromatograms of etheno-acyl-CoAs (and other adenine-containing compounds), prepared from liver extracts of female (A) or male (B) wild type and PMP34 deficient mice on a normal or phytol-enriched diet, spiked with internal standard (500 pmol 3-methyldodecanoyl-CoA, IS). Arrows indicate the elution, based on spiking and co-elution, of phytanoyl-CoA (Ph), pristanoyl-CoA (Pr), and 4,8,12-trimethyltridecanoyl-CoA (3MC13). New peaks appearing in the phytol-treated female +/+ animals, and further increased in all –/– mice, are marked with a red asterisk. Some phytol-derived intermediates, marked with a black asterisk, elute close to straight acyl-CoAs, impeding baseline separation. The elution of different standards as etheno-derivatives, is shown in panel C (taken from Mezzar et al., 2017): mix 1 (red) contains CoA, acetyl-, propionyl-, hexanoyl-, decanoyl-, palmitoyl- and lignoceroyl-CoA; mix 2 (green) acetyl-, octanoyl-, tetradecanoyl-, palmitoyl- and eicosanoyl-CoA; mix 3 (blue), IS and pristanoyl-CoA; mix 4 (black) phytanoyl-CoA. Panel (D) shows the quantification of known and some unknown, labeled as A–C in panel (A), phytol-derived acyl-CoAs (values based on 2 separate mice per gender and per genotype; mean ± SD).