Effects of ALM16 and individual extracts (AM and LE) on the protein expression in hepatic lipid metabolism, CPT-1, SREBP-1c, and FAS, in HFD-fed mice. (a) Western blot analysis of CPT-1, SREBP-1c, and FAS in the liver tissues of HFD-fed mice. The protein expression levels of (b) CPT-1, (c) SREBP-1c, and (d) FAS normalized to β-actin relative to HFD-fed mice (n = 4). Values are presented as mean ± standard error of the mean (SEM). #P < 0.05, ##P < 0.01, and ###P < 0.001 vs. ND group; ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 vs. HFD group. ND: normal diet; HFD: high fat diet; AM, HFD + AM extract (100 mg/kg); LE: HFD + LE extract (100 mg/kg); ALM16-L: HFD + ALM16 (50 mg/kg); ALM16-M: HFD + ALM16 (100 mg/kg); ALM16-H: HFD + ALM16 (200 mg/kg); MT: HFD + milk thistle extract (100 mg/kg).