Figure 5.

miR‐186 directly targets METTL3 and negatively regulates METTL3 expression. A, A total of 6 miRNA (miR‐520b, miR‐186, miR‐372, miR‐302d, miR‐600 and miR‐34c‐3p)‐targeted METTL3 were selected. B, A total of 6 miRNAs (miR‐520b, miR‐186, miR‐372, miR‐302d, miR‐600 and miR‐34c‐3p) were transfected into HuH‐6 cells, and the inhibitory effect on METTL3 expression was evaluated by qRT‐PCR. C, The protein expression levels of METTL3 were assessed by Western blot. D, Bioinformatics analysis predicted miR‐186 binding sites located in the 3’‑UTR of METTL3. E, The mRNA expression levels of METTL3 were assessed by qRT‐PCR. F, WT 3’‐UTR or mutated 3’‐UTR of METTL3 was cloned and constructed into luciferase reporter vector, and then co‐transfected with miR‐186 mimics or NC control into HEK293 cells. Relative luciferase activity was assessed 48 h after transfection. G, The expression of miR‐186 in HB tissues and non‐tumour tissues in GEO database was analysed. H, The expression of miR‐186 in HB tissues and non‐tumour tissues in ZZU cohort was assessed by qRT‐PCR. I, Pearson correlation analysis of the relationship between METTL3 expression level and miR‐186 expression level in ZZU cohort. *P < .05, **P < .01, ***P < .001