Figure 8.

miR‐186/METTL3 axis regulates Wnt/β‐catenin signalling in human HB. A, GO and (B) KEGG analysis showed the enriched biological functions of differentially expressed gene profiles. C, GSVA showed the correlations between METTL3 mRNA levels and signalling pathways. D, E, The protein levels of Wnt/β‐catenin pathway‐related targets in HepG2 or HuH‐6 cells transfected with miR‐186 mimics, METTL3 plasmid, miR‐186 inhibitor, METTL3 siRNA or relative NC. F, Representative immunohistochemical staining images and relative expression levels of Wnt/β‐catenin pathway‐associated proteins in tumours from Lenti‐miR‐186 group and Lenti‐NC group. Scale bars, 200 μm. G, The proposed function model of miR‐186/METTL3 axis in HB