Figure 1.

A20 is inhibited and the R/O ratio is increased under hypoxia, and the R/O ratio is suppressed by A20. A, Cells were treated with different oxygen volume fraction for 24 h. The protein expression of HIF‐1α, A20, OPG and RANKL was measured by Western blot, B and the R/O ratio of mRNA expression by RT‐PCR. C, Cells were incubated in 2% O₂ at different time points. A20, OPG and RANKL expression levels were measured by Western blot, D and the R/O ratio of mRNA expression by RT‐PCR. E and F, The protein expression of A20, OPG and RANKL in cells transfected with A20‐targeted lentivirus for overexpression or silencing for 24 h was detected by Western blot. Data are presented as mean ± SD, *P < .05, **P < .01