Figure 2.

A20 is negatively regulated by autophagy in a time‐dependent manner and can delay the upregulation of R/O ratio. A and B, Cells were transfected with lentivirus A20 for overexpression, followed by treatment in 2% O₂ for 0, 12, 24, 48 and 72 h. The protein expression of A20, TRAF6, ATGs, osteoclast‐related factors and the R/O ratio in the LV‐A20 group was observed by Western blot. C and D, Cells were transfected with lentivirus A20 for silencing, followed by treatment in 2% O₂ at different time points. The protein expression of A20, TRAF6, ATGs, osteoclast‐related factors and the R/O ratio in the si‐A20 group was detected by Western blot. Data are presented as mean ± SD, *P < .05, **P < .01, ***P < .001