Figure 6.

A20 inhibits osteoclastogenesis through downregulating autophagy under hypoxia. A, After becoming pre‐osteoclasts, BMMCs were cultured in CM from cells transfected with lentivirus A20 for silencing. Osteoclast differentiation was measured by TRAP staining. B, The cytoskeleton of multinuclear cells was detected by IF (bar = 100 μm). C, The areas of hydroxyapatite resorption were measured by toluidine blue staining (bar = 100 μm). D, The expression of osteoclast‐related factors (CTSK, MMP9, V‐ATPase D2 and TRAP) in BMMCs was detected by Western blot. Data are presented as mean ± SD, *P < .05, **P < .01, ***P < .001