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. 2020 Feb 6;53(3):e12778. doi: 10.1111/cpr.12778

Figure 7.

Figure 7

A20 suppresses autophagy dependent the regulation of TRAF6 ubiquitination under hypoxia. A, Cells were transfected with lentivirus TRAF6 for silencing and then treated with 2% O₂ for 24 h. Western blot was applied to detect the protein expression of A20, TRAF6, ATGs and osteoclast‐related factors. B, The co‐IP assay was used to identify different types of ubiquitination (K48‐linked and K63‐linked) on TRAF6