Figure 3.

Effects of MitoQ on compression‐induced alterations in mitochondrial dynamics in human NP cells. (A‐B) Human NP cells were pre‐treated with MitoQ (500 nmol/L) for 2 h and then exposed to compression for 36 h. The colocalization of Drp1 and Tom20 was examined by confocal microscopy. Scale bar: 10 μm. (C‐D) The protein levels of mitochondrial Drp1 (mito‐Drp1) and cytoplasmic Drp1 (cyto‐Drp1) in the human NP cells were measured by Western blotting. (D‐F) The protein levels of total Drp1, Mff, Fis1, Mid49, Mid51, Mfn1, Mfn2 and Opa1 in the human NP cells were measured by Western blotting. Data are represented as the mean ± SD. ***P < .001, **P < .01, *P < .05, n = 3