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. 2020 Feb 5;53(3):e12779. doi: 10.1111/cpr.12779

Figure 4.

Figure 4

Effects of MitoQ‐maintained mitochondrial dynamics balance on compression‐induced damage in human NP cells. Human NP cells were pre‐treated with MitoQ (500 nmol/L) for 2 h or Mdivi‐1 (20 μmol/L) for 4 h prior to the compression treatment for 36 h. Before the administration of MitoQ and compression, human NP cells were pre‐treated with 5 μmol/L of FCCP for 30 min. (A‐B) The intracellular ROS levels in the human NP cells were detected using the DCFH‐DA and measured by flow cytometry. (C‐D) The mitochondrial ROS levels were detected using the MitoSOX Red and measured by flow cytometry. (E‐F) Mitochondrial membrane potential was detected by JC‐1 staining and measured by flow cytometry. (G‐H) Annexin V‐APC/7‐AAD staining results showing the rate of apoptosis in human NP cells. (I) Intracellular MDA levels in the human NP cells. (J‐K) The protein levels of caspase 3 and c‐caspase 3 in the human NP cells were measured by Western blotting. Data are represented as the mean ± SD. ***P < .001, **P < .01, *P < .05, n = 3