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. 2020 Feb 5;53(3):e12779. doi: 10.1111/cpr.12779

Figure 5.

Figure 5

Effects of MitoQ on PINK1/Parkin pathway‐mediated mitophagy in human NP cells exposed to compression. (A‐F) Human NP cells were pre‐treated with MitoQ (200 nmol/L, 500 nmol/L) for 2 h and then exposed to compression for 36 h. (A‐C) The protein levels of Parkin and PINK1 in the human NP cells were measured by Western blotting. (D‐F) The protein levels of mito‐Parkin and cyto‐Parkin in the human NP cells were measured by Western blotting. (G‐J) Human NP cells were pre‐treated with MitoQ (500 nmol/L) for 2 h and then exposed to compression for 36 h. (G‐H) The colocalization of Parkin and Tom20 was examined by confocal microscopy. Scale bar: 10 μm. (I‐J) The colocalization of LC3 and Tom20 was examined by confocal microscopy. Scale bar: 10 μm. (K, L) The protein levels of mitochondrial LC3 in the human NP cells were measured by Western blotting. Data are represented as the mean ± SD. ***P < .001, **P < .01, *P < .05, n = 3