Figure 4.

Curcumol inhibits liver sinusoidal endothelial cells (LSECs) angiogenesis by inhibiting Hh signalling pathway. A, Western blot analysis of transcription factor Gli1 expression in nucleus (n = 3). B, Immunofluorescence analysis of Gli1 (n = 3). Scale bar = 20 μm. C, Western blot analysis of HIF‐1α and angiogenic properties of LSECs treated with Hh signalling agonist (SAG 0.3 μmol/L) or inhibitor (cyclopamine 3 μmol/L) (n = 3). D, Tubulogenesis assay was visualized and quantified with ImageJ (n = 3). Scale bar = 500 μm. ^* P < .05, ^** P < .01 and ^*** P < .005 versus control group; ^# P < .05, ^## P < .01 and ^### P < .005 versus model group