Figure 8.

Curcumol inhibits liver sinusoidal endothelial cell angiogenesis through regulating Hh signalling pathway in mice. A, Analysis of sinusoidal fenestrae (arrowhead) in liver tissues (n = 3). Scale bar = 1 μm. B, Immunohistochemical analyses of endothelial markers CD31, CD34 and vWF in liver tissues (n = 3). Scale bar = 100 μm. C, Western blot analysis of endothelial markers and angiogenic factors (n = 3). D, Immunofluorescence analysis of endothelial markers CD31 accompanied by HIF‐1α, VEGF‐A, Smo and PROX1 in liver tissues (n = 3). Scale bar = 20 μm. ^* P < .05, ^** P < .01 and ^*** P < .005 versus control group; ^# P < .05, ^## P < .01 and ^### P < .005 versus model group