Figure 7.

NOTCH2 siRNA partially weakened the pro‐proliferative effect of miR‐29b‐3p inhibitor. A, Cell viability was examined via CCK‐8 assays. Increased cell viability was observed in cells transfected with miR‐29b‐3p inhibitor, and co‐transfection with NOTCH2‐siRNA decreased cell viability. B and C, EdU incorporation assay of cardiomyocytes. Increased EdU incorporation was observed in the miR‐29b‐3p inhibitor group, and co‐transfection with NOTCH2‐siRNA decreased this change (n > 1000, bar = 50 μm). D, WB analysis of NOTCH2, PHH3 and PCNA. E, Relative quantification of NOTCH2, PHH3 and PCNA protein. The expression of NOTCH2, PHH3 and PCNA protein was higher in the miR‐29b‐3p inhibitor group and was decreased by co‐transfection with NOTCH2‐siRNA. The data are presented as the mean ± SEM. Statistical significance is shown as *P < .05 vs controls, **P < .01 vs controls, and ***P < .001 vs controls