Figure 8.

NOTCH2 promoted cardiomyocyte proliferation. A‐C, qRT‐PCR and WB were performed in HL1 cells transfected with pCHD‐NICD2‐puro plasmid. The plasmid increased the endogenous NOTCH2 expression. D, Cell viability was examined via CCK‐8 assays. Cells transfected with pCHD‐NICD2‐puro plasmid promoted cell viability compared with the control group. E and F, EdU incorporation assay of cardiomyocytes. Overexpression of NOTCH2 increased EdU incorporation (n > 1000, bar = 50 μm). G, WB analysis of PHH3 and PCNA. H and I, Relative quantification of PHH3 and PCNA protein. The expression of PHH3 and PCNA protein was up‐regulated in the NOTCH2 group. The data are presented as the mean ± SEM. Statistical significance is shown as *P < .05 vs controls, **P < .01 vs controls, and ***P < .001 vs controls