Figure 9.

NOTCH2 attenuated the inhibitory effect of miR‐29b‐3p mimic on cell proliferation. A, Cell viability was examined via CCK‐8 assays. Decreased cell viability was observed in cells transfected with miR‐29b‐3p mimic, and co‐transfection with NOTCH2 increased cell viability. B and C, EdU incorporation assay of cardiomyocytes. Decreased EdU incorporation was observed in the miR‐29b‐3p mimic group, and co‐transfection with NOTCH2 decreased this change (n > 1000, bar = 50 μm). D, WB analysis of NOTCH2, PHH3 and PCNA. E, Relative quantification of NOTCH2, PHH3 and PCNA protein. The expression of NOTCH2, PHH3 and PCNA protein was lower in the miR‐29b‐3p mimic group and was increased by co‐transfection with NOTCH2. The data are presented as the mean ± SEM. Statistical significance is shown as *P < .05 vs controls, **P < .01 vs controls, and ***P < .001 vs controls