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. 2020 Mar 24;11:469. doi: 10.3389/fmicb.2020.00469

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Copyright © 2020 Ramuta, Starčič Erjavec and Kreft.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rinsing cAM + atb patches in phosphate-buffered saline (PBS) decreases the antimicrobial effect of cAM + atb patches on UPEC DL88 and UPEC DL90 strains. (A–C,G–I) Increased rinsing time of cAM + atb patches results in a decrease of antimicrobial zones in case of UPEC DL88 and UPEC DL90. (D–F,J–L) cAM + atb patches have no antimicrobial effect on UPEC DL88-Gm^r and UPEC DL90-Gm^r. These results were obtained by employing antimicrobial efficiency assay with AM patches on solid agar. Two independent replications of experiments using two biological samples were conducted and three technical repeats were performed for each condition. Square frame – the location of cAM + atb patch, overgrown with tested strain. Scale bars: 10 mm.