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. 2020 Mar 24;11:469. doi: 10.3389/fmicb.2020.00469

FIGURE 6.

FIGURE 6

The range of antimicrobial effect of fresh (fAM) and cAM homogenates varies between tested strains. (A–F) Homogenates of fAM and cAM have antimicrobial effect on S. aureus, UPEC DL94 and Enterobacter sp. The range of antimicrobial effect of fAM and cAM (1 week at –80°C) homogenates varies, namely between strong (S. aureus)/moderate (UPEC DL94)/minor (Enterobacter sp.) antimicrobial effect. Furthermore, fAM homogenate inhibits the growth of tested strains (A–C), while cAM (1 week at –80°C) homogenate inhibits the growth of S. aureus and decreases the growth of UPEC DL94 and Enterobacter sp. (D–F). The quantity of fAM and cAM (1 week at –80°C) homogenates used was 5 μl (upper rows) and 10 μl (lower rows). Scale bars: 10 mm. (G) The average antimicrobial effect of fAM and cAM homogenates on all tested strains. Larger volumes of homogenates (10 μl) have greater antimicrobial effect than smaller volumes (5 μl). For fAM each point represents the mean diameter of the antimicrobial zone ± SE (mm) for three independent replications of experiments using three biological samples; each experiment was performed in six technical repeats for each strain. For cAM (1 week at –80°C) each point represents the mean diameter of the antimicrobial zone ± SE (mm) for four independent replications of experiments using four biological samples; each experiment was performed in three to six technical repeats for each strain. For cAM (10 weeks at –80°C) each point represents the mean diameter of the antimicrobial zone ± SE (mm) for five independent replications of experiments using five biological samples; each experiment was performed in six technical repeats for each strain. For cAM (10 weeks at –20°C) each point represents the mean diameter of the antimicrobial zone ± SE (mm) for one to three independent replications of experiments using one to three biological samples; each experiment was performed in three to six technical repeats for each strain. Bars in red represent the mean diameter of the antimicrobial zone ± standard error (mm) of all tested strains.