Table 3.
Antigen and adjuvant effects on the anti-Salmonella Enteritidis (SE) IgY and anti-Salmonella Typhimurium (ST) IgY titers1
| Main effect | Anti-SE IgY titer (P/N)SE | Anti-ST IgY titer (P/N)ST | |
|---|---|---|---|
| a,bLeast square means within each column for antigen or adjuvant without a common letter are significantly different (P < 0.05). | |||
| 1Values represent the least square means of the entire observation period. (P/N)SE = statistical analysis of anti-SE IgY titers values; (P/N)ST = statistical analysis of anti-ST IgY titers values. | |||
| 2SE-OMP = Salmonella Enteritidis outer membrane proteins extract. | |||
| 3ST-OMP = Salmonella Typhimurium outer membrane proteins extract. | |||
| 4FA = Freund’s adjuvant: Freund complete adjuvant for the first immunization and Freund incomplete adjuvant for the booster immunizations. | |||
| 5IM = immunostimulating complexes matrix. | |||
| Antigen | |||
| SE-OMP2 | 7.50a | — | |
| ST-OMP3 | — | 9.66a | |
| ½SE-OMP + ½ ST-OMP | 7.44a | 9.16b | |
| Adjuvant | |||
| FA4 | 8.22a | 10.91a | |
| IM5 | 6.73b | 7.91b | |
| Source of variations | df | Probability | |
| Antigen | 1 | 0.8843 | 0.0400 |
| Adjuvant | 1 | 0.0031 | <0.0001 |
| Antigen × Adjuvant | 1 | 0.5334 | 0.6213 |
| Sampling time | 11 | <0.0001 | <0.0001 |
| Antigen × time | 11 | 0.8045 | 0.1475 |
| Adjuvant × time | 11 | 0.0002 | <0.0001 |
| Antigen × adjuvant × time | 11 | 0.9648 | 0.5464 |
| Variance parameter estimate | |||
| Hen | 0.514 | 0.383 | |
| Residual | 2.803 | 1.035 | |