Table 1.
Comparison between indirect fluorescent antibody (IFA), direct immunohistochemistry (D-IHC), and reverse transcription-PCR (RT-PCR) results to detect turkey coronavirus from intestine sections (n = 50) obtained from experimental-infected turkey embryos
Samples | IFA | D-IHC | RT-PCR |
---|---|---|---|
Ileum | ++ (43/50) | ++ (45/50)1 | 40/502 |
Ileum-cecal junction | ++++ (50/50) | ++++ (50/50) | 50/50 |
Ceca | ++++ (50/50) | ++++ (50/50) | 50/50 |
The intensity of dark red deposit was arbitrarily rated on a scale of − (undetectable) to ++++ (the pattern present at its highest intensity).
Number of positive samples tested by RT-PCR compared with the total samples.