Table 2.
Detection of turkey coronavirus in experimentally infected turkeys and field samples: evaluation of direct immunohistochemistry (D-IHC) procedure and reverse transcription-PCR relative to indirect fluorescent antibody (IFA) standard procedure
| Item | IFA |
Agreement | Sensitivity1 (%) | Specificity2 (%) | |||
|---|---|---|---|---|---|---|---|
| Positive (+) | Negative (−) | ||||||
| Experimentally infected embryos (n = 150) | D-IHC | + | 143 | 7 | 148/150 (98%) | 98 | 58 |
| − | 2 | 5 | |||||
| RT-PCR | + | 140 | 10 | 145/150 | 96 | 66 | |
| − | 5 | 5 | (96%) | ||||
| Filed samples (n = 300) | D-IHC | + | 255 | 3 | 298/300 (99%) | 85 | 93 |
| − | 42 | 43 | |||||
| RT-PCR | + | 255 | 5 | 300/300 | 86 | 90 | |
| − | 40 | 45 | (100%) | ||||
1
Sensitivity = total positive results/(positive results + false-positive results).
2
Specificity = total negative results/(negative results + false-negative results).