Table 3.
Comparison between indirect fluorescent antibody (IFA), direct immunohistochemistry (D-IHC), and reverse transcription-PCR (RT-PCR) results to detect turkey coronavirus from field samples (n = 100) obtained from commercial turkey poults presenting acute enteritis
Samples | IFA | D-IHC | RT-PCR |
---|---|---|---|
Ileum | ++ (55/100) | ++ (58/100)1 | 60/1002 |
Ileum-cecal junction | ++++ (100/100) | ++++ (100/100) | 100/100 |
Ceca | ++++ (100/100) | ++++ (100/100) | 100/100 |
The intensity of dark red deposit was arbitrarily rated on a scale of − (undetectable) to ++++ (the pattern present at its highest intensity).
Number of positives samples tested by RT-PCR compared with the total samples.