Table 4.
Identification of rotavirus in experimentally inoculated poults
| Experiment no. | Interval1 (DPI) | Method2 | Material tested3 | Group4 |
|||
|---|---|---|---|---|---|---|---|
| A | B | C | D | ||||
| 1 | 5 | EM | IC | + | + | + | ND |
| IS | ND | ND | + | ND | |||
| RT-PCR | IS | + | + | + | ND | ||
| 10 | EM | IC | ND | ND | ND | ND | |
| IS | ND | ND | ND | ND | |||
| RT-PCR | IS | + | + | + | ND | ||
| 15 | EM | IC | ND | ND | ND | ND | |
| IS | ND | ND | ND | ND | |||
| RT-PCR | IS | + | ND | ND | ND | ||
| 2 | 10 | EM | IC | ND | ND | ND | ND |
| IS | ND | ND | ND | ND | |||
| RT-PCR | IS | + | + | ND | ND | ||
| 20 | EM | IC | + | + | + | ND | |
| IS | + | + | + | ND | |||
| RT-PCR | IS | + | + | + | ND | ||
| 3 | 5 | EM | IC | + | + | + | ND |
| IS | + | + | ND | ND | |||
| RT-PCR | IS | + | + | + | ND | ||
| 10 | EM | IC | + | + | + | ND | |
| IS | + | + | ND | ND | |||
| RT-PCR | IS | + | + | + | ND | ||
| 15 | EM | IC | + | + | + | ND | |
| IS | + | ND | ND | ND | |||
| RT-PCR | IS | + | + | + | ND | ||
| 4 | 10 | EM | IC | ND | ND | ND | ND |
| IS | ND | + | ND | ND | |||
| RT-PCR | IS | ND | + | + | ND | ||
| 20 | EM | IC | + | + | + | ND | |
| IS | ND | + | ND | ND | |||
| RT-PCR | IS | + | + | + | ND | ||
| 5 | 5 | EM | IC | + | + | + | ND |
| IS | + | + | ND | ND | |||
| RT-PCR | IS | + | + | + | ND | ||
| 10 | EM | IC | ND | ND | ND | ND | |
| IS | ND | ND | ND | ND | |||
| RT-PCR | IS | + | ND | + | ND | ||
| 15 | EM | IC | ND | ND | ND | ND | |
| IS | ND | ND | ND | ND | |||
| RT-PCR | IS | ND | ND | + | ND | ||
1
DPI = days postinoculation.
2
EM = electron microscopy; RT-PCR = reverse transcription-PCR.
3
IC = pooled intestinal contents; IS = unfiltered supernatant of pooled intestinal contents.
4
A = poults inoculated with unfiltered supernatant; B = poults inoculated with filtered supernatant; C = poults inoculated with sediment; D = poults inoculated with PBS (controls). + = rotavirus detected; ND = rotavirus not detected.