Table 5.
Identification of astrovirus by reverse transcription-PCR in experimentally inoculated poults1
| Experiment no. | Interval2 (DPI) | Material tested3 | Group4 |
|||
|---|---|---|---|---|---|---|
| A | B | C | D | |||
| 1 | 5 | IS | + | + | + | ND |
| 10 | IS | + | + | + | ND | |
| 15 | IS | + | + | + | ND | |
| 2 | 10 | IS | + | + | + | ND |
| 20 | IS | ND | ND | ND | ND | |
| 3 | 5 | IS | + | + | ND | ND |
| 10 | IS | + | + | ND | ND | |
| 15 | IS | + | ND | ND | ND | |
| 4 | 10 | IS | + | ND | ND | ND |
| 20 | IS | ND | ND | ND | ND | |
| 5 | 5 | IS | + | + | + | ND |
| 10 | IS | ND | ND | ND | ND | |
| 15 | IS | ND | ND | ND | ND | |
1
Astrovirus was not detected in intestinal contents by electron microscopy in all 5 experiments.
2
DPI = days postinoculation.
3
IS = unfiltered supernatant of pooled intestinal contents.
4
A = poults inoculated with unfiltered supernatant; B = poults inoculated with filtered supernatant; C = poults inoculated with sediment; D = poults inoculated with PBS (controls). + = astrovirus detected; ND = astrovirus not detected.