Table 2.

Primers sequences used in the PCR and RT-PCR used in the present work, gene target, amplicon generated, and references for each virus.

Virus	Gene target	Primer designation	Sequences	Amplicon	Reference
CAstV	ORF-1b	CAS pol 1F	5′GAYCARCGAATGCGRAGRTTG3′	362 bp	Day et al., 2007
		CAS pol 1R	5′TCAGTGGAAGTGGGKARTCTAC3′
ANV	ORF-1b	ANV Pol 1F	5′GYTGGGCGCYTCYTTTGAYAC3′	473 bp	Day et al., 2007
		ANV Pol 1R	5′CRTTTGCCCKRTARTCTTTRT3′
Avian rotavirus	NSP4	NSP4-F30	5′GTGCGGAAAGATGGAGAAC3′	630 bp	Day et al., 2007
		NSP4-R660	5′GTTGGGGTACCAGGGATTAA3′
Avian reovirus	S4	S4-F13	5′GTGCGTGTTGGAGTTTCCCG3′	1120 bp	Pantin-Jackwood et al., 2008
		S4-R1133	5′TACGCCATCCTAGCTGGA3′
IBV	UTR	UTR 11	5′ATGTCTATCGCCAGGGAAATGTC3′	179 bp	Cavanagh et al., 2002
		UTR 44	5′GGGCGTCCAAGTGCTGTACCC3′
		UTR 31	5′GCTCTAACTCTATACTAGCCTA3′
FAdV-1	Hexon	Hexon A	5′CAARTTCAGRCAGACGGT3′	897 bp	Meulemans et al., 2001
		Hexon B	5′TAGTGATGMCGSGACATCAT3′
ChPV	NS	PVF1	5′TTCTAATAACGATATCACT3′	561 bp	Zsak et al., 2009
		PVR1	5′TTTGCGCTTGCGGTGAAGTCTGGCTCG3′