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. 2017 Jun 15;96(9):3058–3067. doi: 10.3382/ps/pex127

Figure 3.

Figure 3.

Lymphocyte proliferative responses to ConA and LPS. Chickens were i.m. injected with Cy at 100 mg/kg BW for 3 days to induce immunosuppression and oxidative stress. After that, the birds were orally administered TS (5 mg/kg BW) in drinking water for 7 days, and then, intranasally immunized twice with a commercial live bivalent vaccine of NDV and IBV at 2 weeks intervals. Splenocytes were prepared from 6 birds of each group before Cy treatment and 7, 14, 21 days after booster for lymphocyte proliferative assay measured by the MTT method as described in the text. Data were expressed as mean ± S.D. (n = 6). Bars with different letters are statistically different (P < 0.05).