Table 3.

Expression stability values (SV) and standard deviations (SD) of Eucalyptus reference genes calculated by the NormFinder software

1 Group						2 Groups
Leaf		Xylem		All organs		Leaf + xylem
Ranking	SV ± SD	Ranking	SV ± SD	Ranking	SV ± SD	Ranking	SV
Eucons04	0.008 ± 0.010	Eucons27	0.010 ± 0.010	Eucons04	0.017 ± 0.007	Eucons04	0.011
Eucons08	0.018 ± 0.009	Eucons07	0.017 ± 0.010	Eucons08	0.021 ± 0.007	Eucons08	0.016
Eucons32	0.023 ± 0.010	Eucons06	0.023 ± 0.010	Eucons21	0.022 ± 0.008	Eucons21	0.019
Eucons21	0.027 ± 0.011	H2B	0.024 ± 0.011	RibL23A	0.032 ± 0.009	RibL23A	0.020
RibL23A	0.033 ± 0.012	Eucons21	0.025 ± 0.011	Eucons06	0.036 ± 0.009	Eucons06	0.036
GAPDH	0.035 ± 0.013	RibL23A	0.027 ± 0.011	H2B	0.037 ± 0.010	H2B	0.037
Eucons06	0.038 ± 0.014	Eucons04	0.028 ± 0.012	Eucons32	0.047 ± 0.011	Eucons27	0.045
H2B	0.045 ± 0.016	Eucons08	0.029 ± 0.012	GAPDH	0.052 ± 0.012	Eucons32	0.046
Eucons07	0.051 ± 0.017	Euc12	0.036 ± 0.013	Eucons27	0.052 ± 0.012	GAPDH	0.048
At2g28390	0.054 ± 0.018	Eucons32	0.050 ± 0.017	Eucons07	0.060 ± 0.014	Eucons07	0.058
Eucons27	0.059 ± 0.020	GAPDH	0.056 ± 0.019	At2g28390	0.073 ± 0.016	Euc10	0.062
TUA2	0.059 ± 0.020	At2g28390	0.058 ± 0.020	Euc12	0.074 ± 0.016	At2g28390	0.067
Euc12	0.069 ± 0.023	TUA2	0.071 ± 0.023	Eucons43	0.101 ± 0.022	Euc12	0.068
Euc10	0.074 ± 0.024	Eucons43	0.088 ± 0.029	TUA2	0.103 ± 0.022	Eucons43	0.083
Eucons43	0.086 ± 0.028	Euc10	0.138 ± 0.044	Euc10	0.106 ± 0.023	TUA2	0.094
Best combination of two genes						Eucons04 Eucons08	0.009

Complementary DNAs from leaf and xylem (E. grandis, E. dunnii, E. pellita, E. saligna and E. urophylla), xylem (E. globulus) and flower (E. grandis) were subjected to RT–qPCR and results were analyzed with the NormFinder software in single groups of leaf or xylem, or all tissues/organs together. Results for the groups of leaf and xylem together are also presented. The best two reference genes for all the analyses performed are indicated at the bottom.