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. 2019 Jan 22;98(6):2371–2379. doi: 10.3382/ps/pez011

Table 1.

Oligonucleotide primers used for detection of avian respiratory viral pathogens.

Virus Targeting gene Primer Size bp Reference
Avian influenza Matrix M-F TGA TCT TCT TGA AAA TTT GCA 270 (Zakia et al., 2013)
M-R TGT TGA CCA AAT GAC CCT CG
H5 H5-F GAT TGT AGT GTA GCY GGA TGG 400 (Hussein et al., 2015)
H5-R CTT GTC TGC TCT KCM KCA TC
H9 H9-F CTY CAC ACA GAR CAC AAT GG 500 (Lee et al., 2014)
H9-R GTC ACA CTT GTT GTT GTR TC
N8 N8F-ATA GTG ACC GTT GGC TCC ATT TCA 600 This study
N8R- TGT CTA TTG GCT GGA GCG TCA GTC AT
H5 HA1 UNI-RG-5 TAT TCG TCT CAG GGA GCG AAA GCA GG 1123 (Lee and Suarez, 2008)
EGY-H5-R: GCT CGT TGC TAT GGT GGT AC (Shany, 2015)
NDV HA NDV-F ATG GGC TCC AAA CCT TCT ACC AGG 1500 This study
NDV-R ATG CTC TCG TGG TGG CTC TCA TCT GAT
IBV S1 XCE1-CAC TGG TAA TTT TTC TGA TGG 464 (Cavanagh et al., 1999)
XCE1-CTC TAT AAA CAC CCT TAC A
ILT ICP4 ICP4-2F- CTT CAG ACT CCA GCT CAT CTG 636 (Chacon and Ferreira, 2009)
ICP4-2R- AGT CAT GCG TCT ATG GCG TTG AC