Table 3.

Effect of feed matrix and feed additive combination and effect of feed additive on PEDV detection using qRT-PCR^*,†

	Feed additive
Item	Control	EO	BA	EO + BA	SEM	P
Matrix × feed additive
FEED‡	34.5	34.5	34.6	36.5	0.34	0.085
SDPP║	29.1	29.1	29.2	29.6
Treatment	31.8b	31.8b	31.9b	33.0a	0.24	<0.001

^*An initial tissue culture (2.5 mL diluted virus inoculum, 10⁵ TCID₅₀/mL) was inoculated into 22.5 g of gestation diet (FEED) or SDPP treated with 0.02% EO product, 0.5% BA product, combination of EO and BA products (EO + BA) (DSM Nutritional products, Parsippany, NJ), or no chemical treatment. A total of 168 samples were used for the analysis with each treatment represented by a mean of n = 21 for the matrix × treatment interaction, and n = 42 for the main effect of treatment.

^†Ct required to detect genetic material. A higher Ct value is indicative of less genetic material present.

^‡Swine gestation diet.

^║SDPP (APC Functional Proteins, Ankeny, IA).

^a,b,cMeans within item lacking common superscript differ (P < 0.05).