Figure 3.
Inhibitory effects of HY on IBV infection detected by immunofluorescence assays. The CEK cells were infected with 100 TCID50 IBV for 1 h at 37˚C followed by incubation with serially diluted HY at concentrations of 3.125, 6.25, and 12.5 µg/mL for 30 h. The IBV-infected CEK cells, 10 μg/mL RT-treated CEK cells after IBV infection and mock CEK cells were included as controls, respectively. Fluorescence intensity (20×) is proportional to viral infectivity and inversely related to the treatments with different concentrations of HY. CEK = chicken embryo kidney; HY = hypericin; RT = ribavirin.