Table 1.
Sequences of primers used for the qRT-PCR assays.
| Gene | Primer sequence (5′-3′) | Amplicon size (bp) | Annealing temperature (°C) | Accession number |
|---|---|---|---|---|
| IBV | F: CAAGCTAGGTTTAAGCCAGGT | 189 | 58.3 | FJ904723.1 |
| R: TCTGAAAACCGTAGCGGATAT | ||||
| Fas | F: CTTCCACCTGCTCCTCGTCATTG | 174 | 58.5 | NM_001199487.1 |
| R: TTCGTGCAGCACTGACCACTG | ||||
| FasL | F: CCTTCACCAGTGGCATTCAGTACC | 100 | 58.0 | AY765397.1 |
| R: CCTCGTTGTCACAGTGCCTTCC | ||||
| JNK | F: GTTTCCTGATTCTCTCTTCC | 215 | 61.5 | NM_001318225.3 |
| R: CTCCTCAATGGTGTGCTC | ||||
| Bax | F: ACTCTGCTGCTGCTCTCCTCTC | 151 | 58.1 | XM_025145468.1 |
| R: ATCCACGCAGTGCCAGATGTAATC | ||||
| Bcl-2 | F: GAGTTCGGCGGCGTGATGTG | 69 | 60.0 | NM_205339.2 |
| R: TTCAGGTACTCGGTCATCCAGGTG | ||||
| Caspase 3 | F: CCACCGAGATACCGGACTGT | 157 | 61.3 | NM_204725.1 |
| R: AACTGCTTCGCTTGCTGTGA | ||||
| Caspase 8 | F: GGAAGCAGTGCCAGAACTCAGAAG | 151 | 61.0 | AY057939.2 |
| R: TTGTTGTGGTCCATGCACCGATAG | ||||
| β-actin | F: ATTGCTGCGCTCGTTGTT | 189 | 60.0 | K02173.1 |
| R: CTTTTGCTCTGGGCTTCA |