Table 5.

Effects of treatment on PEDV infectivity measured by pig fecal swabs and cecum content by qRT-PCR analysis^a

	PEDV N-gene Real Time-PCR, cycle threshold (Ct)
		Fecal swabs						Cecum contentsd
Item	Feed Ctb	0 dpic	2 dpi	4 dpi	6 dpi	7 dpi	7 dpi
d 0e
PEDV negative	> 40.0	--- f	---	---	---	---	> 45.0
PEDV positive	28.3	---	-- +	+++	+++	+++	22.2
d 1g
PEDV positive	29.7	---	- ++	+++	+++	+++	20.9
0.325% Commercial formaldehydeh	33.0	---	---	---	---	---	> 45.0
1% MCFA (non-aerosolized)i	38.3	---	---	---	---	---	> 45.0
0.66 % Caproic acid	35.0	---	---	---	---	---	> 45.0
0.66% Caprylic acid	35.7	---	---	---	---	---	> 45.0
0.66% Capric acid	30.7	---	---	---	---	---	> 45.0
0.66 % Lauric acid	30.7	---	---	+++	+++	+-+	28.4
0.3% FRA C12j	30.7	---	---	+++	+++	+++	30.2
1% Canola oil	30.7	---	---	+++	+++	+++	20.3
1% Choice white grease	30.0	---	---	+++	+++	+++	15.3
1% Coconut oil	30.3	---	---	---	---	+-+	42.1
1% Palm kernel oil	30.0	---	---	+++	+++	+++	22.1
1% Soy oil	30.0	---	---	+++	+++	+++	24.0

^aAn initial tissue culture containing 10⁶ TCID₅₀/mL PEDV was diluted to 10⁵ TCID₅₀/mL PEDV. Each treatment was inoculated with the 10⁵ TCID₅₀/mL PEDV resulting in 10⁴ TCID₅₀/g PEDV inoculated feed matrix. Three feed samples per day and treatment were collected and diluted in PBS. The supernatant from each sample was then collected for pig bioassay. The supernatant was administered one time via oral gavage on d 0 to each of three pigs per treatment (10 mL per pig). Thus, the cecum contents are represented by a mean of 3 pigs per treatment. Pigs were inoculated at d 12 age.

^bA cycle threshold (Ct of > 40) was considered negative for the presence of PEDV RNA. Feed Ct analysis via qRT-PCR was carried out at Kansas State University. Values are from each analysis day by treatment interaction.

^cDay post inoculation.

^dA cycle threshold (Ct of > 45) was considered negative for the presence of PEDV RNA. Cecum content analysis via qRT-PCR was carried out at Iowa State University Veterinary Diagnostic Laboratory at necropsy of the bioassay. Each value is represented by an n of 3 pigs.

^eD 0 samples are represented from the d 0 analysis day and collected during the qRT-PCR analysis. The samples were collected and kept at −80°C until given to a 12 d old pig via oral gavage.

^f In each instance a (–) signals a negative pig in the bioassay and a (+) represents a positive fecal swab in the bioassay. Each day post inoculation within each treatment has three symbols within each row and column which represents one of the three pigs in each treatment.

^gD 1 samples are represented from the d 1 analysis day and collected during the qRT-PCR analysis. The samples were collected and kept at −80°C until given to a 12 d old pig via oral gavage.

^hKemin Industries, Des Moines, IA.

ⁱMCFA blend of 1:1:1 ratio of caproic, caprylic, and capric acids added directly into the mixer with no atomizing nozzle.

^jFramelco, Raamsdonksveer, Netherlands.