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. 2015 Jul 3;147(1):255–269. doi: 10.1093/toxsci/kfv127

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© The Author 2015. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com

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FIG. 1. — Western blot analysis of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expression in lipopolysaccharide (LPS)-stimulated primary macrophages is reported (A). Densitometric analysis of protein bands of iNOS (B) and COX-2 (C) was performed on 3 separate experiments. GAPDH protein immunoblot was performed to ensure equal sample loading. NO²⁻ (D), IL-6 (E), TNFα (F) and MCP-1 (G) levels were also reported. Lysates and supernatants were obtained from control and 24 h-treated cells with 300 nM polychlorinated biphenyls (PCBs), differently associated, in the presence of LPS (1 µg/ml) stimulus. NO^-2 values (µM) and cytokine levels (ng or pg/ml) are means ± SEM of 3 determinations. As specified in the Materials and Methods section, when PCBs were used in combination, final concentration of pollutants was always 300 nM. ***P < .001 versus control cells; ^##P < .01, and ^###P < .001 versus LPS-treated cells.