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. 2015 Jul 3;147(1):255–269. doi: 10.1093/toxsci/kfv127

FIG. 5.

FIG. 5.

Effects of polychlorinated biphenyl (PCB) 101, 153, and 180, alone (A) or in combination (B), in the presence of lipopolysaccharide (LPS) stimulus, on COX-2 protein expression. Lysates were obtained from control and 24 h-treated cells with PCBs at 300 nM, alone or differently combined, in the presence of LPS (10 ng/ml). Representative immunoblots are shown. Densitometric analysis of protein bands was performed on 3 separate experiments. GAPDH protein immunoblot was performed to ensure equal sample loading. As specified in the Materials and Methods section, when PCBs were used in combination, final concentration of pollutants was always 300 nM. ***P < .001 versus control cells; ###P < .001 versus LPS-treated cells.