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. 2013 Sep 24;209(9):1331–1342. doi: 10.1093/infdis/jit504

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© The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

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Figure 1. — Middle East respiratory syndrome coronavirus (MERS-CoV) replication kinetics in human monocyte–derived macrophages (MDMs), compared with SARS coronavirus (SARS-CoV). MERS-CoV or SARS-CoV was inoculated onto MDMs at 2 tissue culture infective doses (TCID₅₀) per cell. At the indicated hours after infection, cell lysate (A) and culture supernatant (B) were collected to detect the levels of positive-strand viral RNA by reverse-transcription quantitative polymerase chain reaction. Viral titration of the culture supernatants was performed with a TCID₅₀ assay (C). The results of 3 representative donors are demonstrated.