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. 2013 Sep 24;209(9):1331–1342. doi: 10.1093/infdis/jit504

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© The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

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Figure 3. — Cell viability of Middle East respiratory syndrome coronavirus (MERS-CoV)–infected and SARS coronavirus (SARS-CoV)–infected human monocyte–derived macrophages (MDMs). MDMs were seeded at 1.5 × 10⁵/well in a 24-well plate and inoculated with MERS-CoV or SARS-CoV at 2 median tissue culture infective doses per cell or subjected to mock infection. Before infection and 48 hours after infection, MDMs were trypsinized, stained with trypan blue, and counted in duplicate with disposable hemocytometers. Viability represents the ratio of viable cell number of inoculated MDMs 48 hours after infection, compared with that before infection. Results are the mean and standard error of the mean of at least 3 representative donors. *P < .05, by the Student t test.