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. 2015 Apr 22;212(8):1214–1221. doi: 10.1093/infdis/jiv246

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© The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com

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Figure 3. — Luciferase activity of the putative enhancer. The putative enhancer region containing rs383510 was inserted in a luciferase reporter vector. Two constructs representing rs383510 genotype TT and genotype CC, as well as unmodified vector pGL3-Basic, were transfected into A549 cells. A Renilla luciferase vector pGL4.70[hRluc] was cotransfected as an internal control, and luciferase assay was performed 32 hours after transfection. The relative luciferase activity (ratio of firefly to Renilla luminescence) is presented as the mean and SD of triplicated transfection in a representative experiment.