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. 2019 Feb 4;219(12):2015–2025. doi: 10.1093/infdis/jiz029

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© The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

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Figure 3. — Characterization of the immune cell profile in the brain after Rocio virus (ROCV) infection. Six-week-old female C57BL/6 mice (n = 3–6) were inoculated intraperitoneally with 2.8 × 10⁶ plaque-forming units (PFU) of ROCV or medium. Brains were collected 7 days after infection, and the total leukocyte population was purified. Flow cytometry plots indicate gating strategies and percentages of total leukocytes (CD45⁺); A), macrophages (CD11b^highCD45^high) and microglia (CD11b^highCD45^low); B), neutrophils (Ly6G⁺CD45⁺) cells in left 2 panels and myeloperoxidase levels in the right panel; C), and T cells (CD4⁺/CD8⁺); D). **P < .01 and ***P < .001, by the Mann-Whitney test, for comparison of infected and uninfected mice.