Table 1.
The MERS-CoV-Neutralizing Human Monoclonal Antibodies Repertoires From B Cells
| mAb ID | Secondary IDa | Strategy | HV Gene | HD Gene | HJ Gene | HCDR 3 | SHM | V-Region Identity % (nt) | LV/K Gene | LJ Gene | LCDR 3 | SHM | V-Region Identity % (nt) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ab8 | H8-7/L8-15 | B cells of patient | 1-69*04 | 3-10*01 | 3*02 | 18 | 0 | 100.00 | 1-40*01 | 2*01 | 11 | 1 | 98.96 |
| Ab27 | H27-8/L27-17 | B cells of patient | 1-69*09 | 3-10*01 | 3*02 | 18 | 2 | 97.92 | 1-40*01 | 2*01 | 11 | 1 | 98.96 |
| Ab28 | H28-6/L28-13 | B cells of patient | 1-69*09 | 3-10*01 | 3*02 | 18 | 3 | 98.26 | 1-51*01 | 2*01/3*01 | 11 | 3 | 97.89 |
| Ab33-1 | H33-1/L33-7 | B cells of patient | 1-69*09 | 3-10*01 | 3*02 | 18 | 0 | 99.65 | 1-40*01 | 2*01 | 11 | 1 | 99.86 |
| Ab33-2 | H33-1/L33-12 | B cells of patient | 1-69*09 | 3-10*01 | 3*02 | 18 | 0 | 99.65 | 1-40*02 | 2*01 | 12 | 0 | 97.22 |
| Ab38 | H38-7/L38-20 | B cells of patient | 1-69*04 | 3-10*01 | 3*02 | 18 | 0 | 100.00 | 2-14*01 | 2*01 | 10 | 11 | 90.46 |
| Ab39-1 | H39-9/L39-12 | B cells of patient | 1-69*09 | 3-10*01 | 3*02 | 18 | 1 | 98.61 | 1-40*02 | 2*01 | 12 | 0 | 97.22 |
| Ab39-2 | H39-9/L39-13 | B cells of patient | 1-69*09 | 3-10*01 | 3*02 | 18 | 1 | 98.61 | 1-50*01 | 1*01 | 11 | 4 | 93.75 |
| Ab40 | H40-2/K40-2 | B cells of patient | 1-69*04 | 4-17*01 | 6*02 | 16 | 0 | 99.31 | 2-28*01 | 4*01 | 9 | 0 | 99.66 |
| Ab41 | H41-5/K41-6 | B cells of patient | 1-69*09 | 4-17*01 | 6*02 | 16 | 0 | 99.65 | 2-28*01 | 4*01 | 9 | 0 | 99.87 |
| Ab42 | H42-6/L42-8 | B cells of patient | 1-69*09 | 3-10*01 | 3*02 | 18 | 3 | 98.26 | 1-51*01 | 2*01/3*01 | 11 | 3 | 97.54 |
| Ab43 | H43-9/L43-2 | B cells of patient | 1-69*09 | 3-10*01 | 3*02 | 18 | 1 | 98.61 | 1-40*02 | 2*01 | 12 | 0 | 97.22 |
| Ab45 | H45-8/L45-13 | B cells of patient | 1-69*09 | 3-10*01 | 3*02 | 18 | 0 | 99.65 | 2-14*01 | 2*01 | 10 | 11 | 90.52 |
| m336 | 922664705-10 | Phage display library | 1-69*06 | 2-2*03 | 3*02 | 18 | 4 | NA | 1-17*01 | 4*01 | 9 | 2 | NA |
| MCA1 | 1200169277-9 | Phage display of patient’s PBMC | 1-69 | 2-2*01 | 3*02 | 17 | 0 | NA | 3-20*01 | 5*01 | 9 | 0 | NA |
| LCA60 | NA | B cells of patient | 3-15*01 | NA | NA | 18 | 17 | NA | 2-23*01 | NA | NA | 12 | NA |
| MERS-4 | NA | scFv-yeast display library | 3-30-3*01 | 6-19*01 | 4*02 | 7 | NA | 94.5 | 1-47*01 | 17*01 | 13 | NA | 98.0 |
| MERS-27 | NA | scFv-yeast display library | 3-11*03 | 3-3*01 | 1*01 | 16 | NA | 93.9 | 1D-33*01 | 5*01 | 10 | NA | 93.0 |
| Seven nAbs | NA | Phage display library | 1-69*06/09, 3-30*03, 1-3*01 | 2-2*01, 3-10*01, 3-10*02,4-17*01, 4-23*01 | 3*02, 4*02 | 11, 13, 14, 16, 18, 19 | 0–10 | NA | 3-1*01, 1-47*01, 3-15*01, 4-1*01, 3-20*01, 10-45*01 | 1*01, 2*01, 3*01, 3*02, 4*01 | 9, 11 | 2–9 | NA |
| 4C2b | 939186871/4 | Immunized mice | 5-6-4*01 | NA | 2*01 | 9 | NA | NA | 10–96*01 | 1*01 | 9 | NA | NA |
Abbreviations: CoV, coronavirus; ID, identification; mAb, monoclonal antibody; MERS, Middle East respiratory syndrome; NA, not available; nAbs, neutralizing antibodies; nt, nucleotide; PBMC, peripheral blood mononuclear cells; SHM, somatic hypermutation.
aThe origin of light and heavy chains.
bHumanized mAb.