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. 2018 Aug 22;11(5):395–407. doi: 10.1093/jmcb/mjy045

Figure 1.

Figure 1

IFN signal pathway increases P2Y13 expression. (A) Mouse BMDMs were treated with IFN-α, IFN-β, or IFN-γ (100 ng/ml) for 2 h, and mRNA expression of P2Y family was detected by Q-PCR. (B) Mouse PEMs were treated with IFN-β (100 ng/ml) for 4 or 8 h, and the expression of P2Y13 protein was detected by western blotting. (C) Mouse BMDMs were pretreated or not with anti-IFN-α/β receptor IgG (10 μg/ml) for 4 h before being treated with IFN-β (100 ng/ml) for 4 h, and the expression of P2Y13 mRNA was detected by Q-PCR. (D) Mouse PEMs were pretreated or not with Ruxolitinib (10 μg/ml) for 1 h and then stimulated with IFN-β (100 ng/ml) for 4 h. The expression of ISG15 and P2Y13 mRNA was detected by Q-PCR. (E) Mouse PEMs were pretreated with or without fludarabine (100 μg/ml) for 30 min and then stimulated with IFN-β (100 ng/ml) for 4 h. The expression of ISG15 and P2Y13 mRNA was detected by Q-PCR. (F) Mouse PEMs were pretreated or not with fludarabine (100 μg/ml) for 30 min and then infected with VSV (MOI = 1) for 12 h. The expression of P2Y13 mRNA was detected by Q-PCR. (G) TLR3+/+ and TLR3−/− mouse PEMs were treated with poly(I:C) (20 μg/ml) for 4 h. The expression of IFN-β, ISG15, and P2Y13 mRNA was detected by Q-PCR. Data are shown as mean ± SD. **P < 0.01; ***P < 0.001.