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. Author manuscript; available in PMC: 2020 Mar 31.
Published in final edited form as: Oncogene. 2019 Sep 9;39(3):560–573. doi: 10.1038/s41388-019-0975-3

Figure 7. OGA cooperates with OGT to regulate PKM2 O-GlcNAcylation.

Figure 7.

(A) HeLa cells were transiently transfected with the indicated plasmids and treated with 25 mM glucose for 4 hours. HN-PKM2 was immunoprecipitated and analyzed by Western blotting with the indicated antibodies. (B) Working model. Under nutrient-limiting conditions, the O-GlcNAcase activity of OGA targets and activates PKM2, thereby increasing carbon flow into the TCA cycle and promoting cell survival. Under nutrient-rich conditions, the acetyltransferase activity associated with OGA targets and inactivates PKM2 through enhanced OGT-mediated O-GlcNAcylation, thereby increasing aerobic glycolysis and promoting cell proliferation.